In the previous work , we cloned sh2a gene in this region by exon trapping and exon linking , which is a novel member of sh2 family . sh2 domain is considered as " protein recognized code " 在前期工作中,運用外顯子捕獲和外顯子拼接等策略,我們在這一區(qū)域克隆了sh _ 2a基因,是sh _ 2信號蛋白家族的新成員。
Normal tissues and tumor tissues methods a gene was cloned by exon trapping and exon linking technique . homologous analysis of the gene was researched by blast . then we explored the gene expression pattern in various human tissues by northern blot and rt - pcr 正常組織及腫瘤組織實驗方法利用捕獲的外顯于進行blast同源性分析,之后采用外顯于拼接技術克隆新基因,利用rtpcr及northern印跡雜交檢測該基因的組織表達情況,同時以p 《 ctin作內對照。
百科解釋
Exon trapping is a molecular biology technique to identify potential exons in a fragment of eukaryote DNA of unknown intron-exon structure.Duyk, G.